Immunomodulatory Effects of Origanum vulgare L. and Origanum Majorana L. Ethanolic Extracts in Vitro

Background: Many plants possess therapeutic properties against infectious and non-infectious diseases, including cancer inflammatory diseases. Plants may have immunomodulatory properties, too. In this study, we evaluated the effects toxic of ethanolic extract from Lamiaceae family on macrophage, lymphocyte, B Cell Leukemia (BCL-1) in vitro. Materials Methods: Plant samples were purchased, their leaves was prepared by dissolving them ethanol (maceration) for 48 hours. The obtained dried at room temperature. normal lymphocytes macrophages extracted BALB/c mice BCL-1 cultured with different concentrations extracts 24 MTT assay performed to evaluate proliferation, Lactic Dehydrogenase (LDH)-based cytotoxicity measured. Results: We found that a concentration 1 mg/mL significantly increased lymphocyte proliferation (47% Origanum vulgare 95% majorana compared control group). Also, activity 35% after O. administration 23% dose mg/mL. Furthermore, LDH results prove (1 mg/mL) had no effect macrophages. finding showed (with IC50 1.04 1.6 could suppress growth BCL-1. Conclusion: activities dose-dependent manner. tested significant anticancer activity.